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Stroke markers were increased in PTI mice compared to Con. (A) <t>ELISA</t> <t>evaluated</t> <t>S100B</t> and (B) NT-pro BNP. Con (n = 3), Sed-PTI (n = 4), and EP-PTI (n = 4). (C) Photomicrographs depicting Nissl staining of mice brains. Scale bar = 1 mm. (D) Representative images of IF staining at a magnification of 20×. Scale bar = 50 µm. (E) Percentage of GFAP-positive cells. Con (n = 3), Sed-PTI (n = 3), and EP-PTI (n = 3). Data were presented as mean ± SD (*p < 0.05). Con: Control; EP-PTI: Exercised preconditioning-photothrombotic ischemic stroke; Sed-PTI: Sedentary-photothrombotic ischemic stroke.
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Stroke markers were increased in PTI mice compared to Con. (A) <t>ELISA</t> <t>evaluated</t> <t>S100B</t> and (B) NT-pro BNP. Con (n = 3), Sed-PTI (n = 4), and EP-PTI (n = 4). (C) Photomicrographs depicting Nissl staining of mice brains. Scale bar = 1 mm. (D) Representative images of IF staining at a magnification of 20×. Scale bar = 50 µm. (E) Percentage of GFAP-positive cells. Con (n = 3), Sed-PTI (n = 3), and EP-PTI (n = 3). Data were presented as mean ± SD (*p < 0.05). Con: Control; EP-PTI: Exercised preconditioning-photothrombotic ischemic stroke; Sed-PTI: Sedentary-photothrombotic ischemic stroke.
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Stroke markers were increased in PTI mice compared to Con. (A) <t>ELISA</t> <t>evaluated</t> <t>S100B</t> and (B) NT-pro BNP. Con (n = 3), Sed-PTI (n = 4), and EP-PTI (n = 4). (C) Photomicrographs depicting Nissl staining of mice brains. Scale bar = 1 mm. (D) Representative images of IF staining at a magnification of 20×. Scale bar = 50 µm. (E) Percentage of GFAP-positive cells. Con (n = 3), Sed-PTI (n = 3), and EP-PTI (n = 3). Data were presented as mean ± SD (*p < 0.05). Con: Control; EP-PTI: Exercised preconditioning-photothrombotic ischemic stroke; Sed-PTI: Sedentary-photothrombotic ischemic stroke.
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Stroke markers were increased in PTI mice compared to Con. (A) ELISA evaluated S100B and (B) NT-pro BNP. Con (n = 3), Sed-PTI (n = 4), and EP-PTI (n = 4). (C) Photomicrographs depicting Nissl staining of mice brains. Scale bar = 1 mm. (D) Representative images of IF staining at a magnification of 20×. Scale bar = 50 µm. (E) Percentage of GFAP-positive cells. Con (n = 3), Sed-PTI (n = 3), and EP-PTI (n = 3). Data were presented as mean ± SD (*p < 0.05). Con: Control; EP-PTI: Exercised preconditioning-photothrombotic ischemic stroke; Sed-PTI: Sedentary-photothrombotic ischemic stroke.

Journal: Frontiers in Physiology

Article Title: Exercise preconditioning alleviates photothrombotic ischemic stroke in mice by orchestrating neutrophils

doi: 10.3389/fphys.2025.1580283

Figure Lengend Snippet: Stroke markers were increased in PTI mice compared to Con. (A) ELISA evaluated S100B and (B) NT-pro BNP. Con (n = 3), Sed-PTI (n = 4), and EP-PTI (n = 4). (C) Photomicrographs depicting Nissl staining of mice brains. Scale bar = 1 mm. (D) Representative images of IF staining at a magnification of 20×. Scale bar = 50 µm. (E) Percentage of GFAP-positive cells. Con (n = 3), Sed-PTI (n = 3), and EP-PTI (n = 3). Data were presented as mean ± SD (*p < 0.05). Con: Control; EP-PTI: Exercised preconditioning-photothrombotic ischemic stroke; Sed-PTI: Sedentary-photothrombotic ischemic stroke.

Article Snippet: The following ELISA kits were used: S100 calcium-binding protein B (S100B) (1:50, Elabscience, Wuhan, China), N-terminal pro-b-type natriuretic peptide (NT-pro BNP) (1:5, Elabscience, Wuhan, China), and citrullinated histone H3 (CitH3) (1:5, Cayman, Ann Arbor, United States).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Control